The basic objectives of this project are fundamental studies on the interaction between femtosecond laser pulses and glassy materials to realize nano-scale optical structures/waveguides and micro-fluidic devices on the same substrate or basic optical platform, thereby producing “micro-fluidic chips”. The fabrication of intricate microstructures and channels within the bulk volume or surface of optically transparent glassy materials is widely recognized as an important development in the innovation of advanced components in the two apparently distinct areas of photonics and fluidics, with a direct impact to bio-photonics. However, due to incompatibilities in any manufacturing processes, it is difficult to mix these two functionalities. The processing of glass with femtosecond pulses is a versatile technique, which offers unique capabilities for the fabrication of micro-devices that integrate on a monolithic substrate micro-optics with micro-fluidics. However, it is equally important to develop the analytical tools to study the fluid flow in the micro-fluidic circuitry, and we shall also focus our efforts towards this goal.